InSphero life science company head quartered in Switzerland.
“The 3D InSight™ Gastrointestinal Organoid model is based on patient-derived human small intestine tissue. The model originates from ethically sourced biopsies and is expanded as long-term organoid cultures. In addition to the small intestine model, our biobank includes organoids derived from colon, rectum, and stomach tissue. These additional GI segment models are currently available for custom projects.”
“3D InSightTM Small Intestine Organoid model represents a physiologically relevant “mini-gut” and contains the key epithelial cell types found in native intestinal tissue. These include enterocytes, goblet cells, Paneth cells, and enteroendocrine cells. The presence of these cell populations is confirmed through gene expression analysis and immunostaining.”
“Organoids formation on Gri3D plates is driven by the sedimentation of uniformly distributed single cells into hydrogel microcavities without solid ECM. There cells aggregate and form organoids within each microwell with a dilute matrix cue that triggers polarization.”
“The 3D InSight™ Small Intestine Organoid Model contains a mix population of both proliferative stem-cell and differentiated intestinal cell types. This balance enables physiologically relevant responses while maintaining proliferative capacity of the organoid.”
“Each microtissue is a triple-culture model containing iPS-derived differentiated astrocytes, Glutamatergic (excitatory) neurons, and GABAergic (inhibitory) neurons.”
“Yes. 3D InSight™ Human Neural Microtissues remain viable and functionally active for up to 8 weeks after aggregation, providing an assay window of up to 4 weeks upon delivery. This enables long-term compound exposure studies designed to better reflect clinical dosing regimens.”
“The microtissues are designed for a wide range of applications, including CNS safety assessment, neurotoxicity screening, functional pharmacology, long-term drug treatment studies and mechanism-of-action investigations.”
“Calcium imaging in neural microtissues measures changes in intracellular calcium as a functional readout of synchronous neuronal activity, making it well suited for scalable screening. MEA studies directly record electrical activity, providing deeper insights into firing patterns, bursting behavior, network connectivity, and mechanism of action.”
“The balanced model contains 50% neurons and 50% astrocytes, reflecting cortical glia-to-neuron proportions. The model is suited for applications such as general neurotoxicity screening, neurodegenerative disease modeling, metabolic profiling, or neuroinflammation studies.”
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